Fig. 5. PIPkin Ib stimulates adhesion. (A) Following transfection with GFP-tagged PIPkin I and (and derivatives) and the GFP-tagged PH domain for PLC1, the level of adhesion to serum-coated plastic was quantified and is expressed as a percentage difference to non-transfected cells. Using a Student's t-test, a P value of <0.01% was obtained (*) between these transfectants. (B) The dose-response for increasing levels of PIPkin I transfection derived from the total levels observed in (A). `Low', `medium', `high' and `very high' correspond to arbitrary divisions in the level of expression. The levels of expression were determined by the quantity of GFP fluorescence detected by a FACs instrument. The level of adhesion for each individual transfectant was obtained from an average of three experiments and error bars represent the standard deviation from the mean. Using a Student's t-test, a P value of <0.01% was obtained (*) between these transfectants.