Fig. 2. EGF accumulates in aberrant early endosomes in TSG101-depleted cells. (A-C) Lamin A-depleted HeLa cells were allowed to internalise Alexa Fluor488-labelled EGF for 3 hours and cells were examined by fluorescence microscopy for EEA1 (A), Hrs (B) or LAMP-2 (C) (note that virtually all the Alexa Fluor488-labelled EGF had been lost from cells at this time, and panels showing the residual Alexa Fluor488-labelled EGF are not provided). (D-L) TSG101-depleted cells were allowed to internalise Alexa Fluor488-labelled EGF for 3 hours. Cells were examined by fluorescence microscopy for EGF (D,G,J; green), EEA1 (E; red), Hrs (H; red) or LAMP-2 (K; red). Merged images are shown in F,I,L. Bar, 10 µm.