Fig. 7. Transport to lysosomes is impaired in TSG101-depleted cells. (A) TSG101-depleted HeLa cells were incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 3 hours, with TfHRP added for the final 30 minutes. Examples of EGFR-gold complexes are indicated by arrows. (B,C) Lamin A-depleted control (B) or TSG101-depleted (C) HeLa cells were incubated for 8 hours with fluid-phase HRP at 37°C. Similar results were obtained after 4 and 6 hours (not shown). Electron dense content is crosslinked HRP. Lysosomes (based on morphology) are indicated by arrowheads. Bars, 0.5 µm.