(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Effect of the MTP18 C-terminus on protein localisation and mitochondrial morphology. (A) (left) diagram of myc-tagged (C-terminal red boxes) MTP18 wild-type and mutants that lack the N-terminus (including the predicted TM1) or the C-terminus (including the predicted TM2 and TM3). Transient protein expression was confirmed by western blot (right panel) probed with anti-MTP18 (*, endogenous MTP18; arrow, full-length MTP18–myc; arrowhead, truncated protein variants. (B) Representative confocal immunofluorescence images of transiently transfected COS-7 cells expressing wild-type (upper row), N-terminally truncated (middle row) and C-terminally truncated MTP18 (lower row). Shown is distribution of MTP18 (green, anti-MTP18) and mitochondrial morphology (red, anti-Hsp60). Bars, 20 µm. (C) Immunofluorescence staining of {Delta}91-166-MTP18-myc in transfected COS-7 cells with anti-MTP18 (green) and anti-Hsp60 (red) to investigate its subcellular localisation. Arrowheads, untransfected cells; arrow, cell with low expression levels of {Delta}91-166-MTP18-myc; double-arrow, cell with high expression levels of {Delta}91-166-MTP18-myc.