Fig. 11. Tf accumulates in Rab11-positive recycling endosomes. Clone 9 cells were transiently transfected with GFP-Rab11. After 24 h of transfection, the cells were then treated with (A) DMSO as a solvent control or (B) 20 µM CI-976 for 10 minutes at 37°C prior to labeling with Alexa568-Tf for 45 minutes at 37°C. Arrows indicate juxtanuclear cluster of vesicles that contain both GFP-Rab11 and Alexa568-Tf, as evidenced by the yellow merged images in B1-B3.