Fig. 2. Tubules can form independently from Golgi subcompartments following CI-976 treatment. Clone 9 rat hepatocytes were treated with solvent control (A,B) or 20 µM CI-976 (C-H) for various amounts of time as indicated. Cells were then fixed and processed for double-immunofluorescence staining of Golgi marker proteins, ManII (A,C,E,G) and TGN38 (B,D,F,H).