Fig. 3. TGN and early endosomes do not fuse. Clone 9 cells were treated with solvent control (A) or 20 µM CI-976 (B) for 50 minutes. Cells were then labeled with 1 mg ml^–1 Alexa568-dextran for 5 minutes prior to fixing and staining for immunofluorescence localization of M6PR. The sets of three images (A1-A3 and B1-B3) are from consecutive slices in the Z plane taken using a spinning disc confocal microscope (Perkin-Elmer).