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Fig. 4. P2X4-GFP mutants Y372A, Y378A and Y378F, but not Y372F or V375A, show internalization defects. Representative confocal micrographs of P2X4-GFP and related mutants (Y372A, Y372F, V375A, Y378A and Y378F) expressed in HEK293 cells. Nucleic acids are shown in red. Internalization-deficient mutants (Y372A, Y378A, Y378F) have greater plasma membrane expression. Bar, 10 µm.