Fig. 3. The hydrophobic subdomain determines the trafficking pathways of NPP1 and NPP2. (A) Localization in HEK293 cells of NPP1, NPP2, NPP1-2_12-30-1 and NPP2-1_59-79-2, each expressed as fusions with an N-terminal HA-tag and a C-terminal Myc-tag, and detected by confocal fluorescence microscopy with anti-Myc antibodies. (B) An aliquot of the same cells was lysed for immunoblotting with anti-HA and anti-Myc antibodies. All data shown are representative for at least three independent experiments.