Fig. 7. Cellular and secreted NPP2 differ in their extent of glycosylation. HA-NPP2-Myc was expressed in HEK293 cells with or without ₁-PDX, as indicated. The cell lysates and media were processed for immunoblotting with anti-Myc antibodies in the absence or presence of 50 mM 2-mercaptoethanol and before or after a pretreatment for 50 mU of N-glycosidase F, as indicated. The data are representative for at least three independent experiments.