Fig. 5. Colocalization of mitochondrial and lyosomal markers on nutrient depletion in controls and LAMP1 (LA1) or LAMP2 (LA2) knockdown cells. HeLa cells were treated with siRNA specific for emerin, LAMP1 and/or LAMP2 (24 hours), then transfected with mt-DsRed (which localizes to mitochondria) and SytVII-GFP (which localizes to lysosomes), cultured for 24 hours and then incubated in CM or NF medium for 4 hours, followed by confocal microscopy as in Fig. 3. Representative cells are shown in A and the degree of colocalization (X±s.d., n=50) between mt-DsRed and SytVII-GFP is shown in B. The graphs (right panels) represent the fluorescence distribution determined for section of the cells, as indicated by the orientation of the arrow. Bar, 10 µm.