Fig. 6. Colocalization of mt-DsRed and SytVII-GFP in wild-type (WT) and LAMP1^–/– LAMP2^–/– (DKO) MEF. The degree of colocalization was measured similarly as in Fig. 4, before and after overnight starvation of amino acids and serum. Examples of the normal separation of mitochondria and lysosomes are shown in the upper part of A, whereas examples of how these organelles colocalize on starvation in WT but not DKO cells are shown in the middle and lower parts of A, respectively. Bar, 10 µm. The graphs (right panels) represent the fluorescence distribution determined for section of the cells, as indicated by the orientation of the arrow. Quantitative data are shown in B.