Fig. 8. Effect of mitochondrial apoptosis inhibitors on vacuolization-associated death. (A,B) Effect of Bcl-2 and vMIA on cell death. HeLa cells stably transfected with Bcl-2 or vMIA were subjected to the indicated LAMP-specific siRNAs, and the frequence of _m^low (A) or dead (B) cells was measured by staining with DiOC₆(3) or PI, respectively. (C) Accumulation of the autophagic vacuole marker LC3-GFP in vesicles from Neo controls, Bcl-2-transfected and vMIA-transfected HeLa cells or Neo control vector-only transfected cells treated with Z-VAD-fmk. Note that none of the apoptosis inhibitors influenced the vacuolar accumulation of LC3-GFP. Bar, 10 µm.