Fig. 4. N-WASP pits do not incorporate WAVE2. (A,B) Representative examples of cells co-expressing EGFP-tagged N-WASP (green in merged images) and mRFP-WAVE2 (red in merged images) analysed by TIRFM. (A) N-WASP readily accumulates in pit-like structures, which were entirely devoid of detectable WAVE2. Arrowheads indicate examples for pit-like N-WASP-containing structures disappearing in the course of the experiment. Arrow indicates pit-like structure that has appeared in the same time period. None of these structures displayed detectable WAVE2 enrichment. (B) Episodes of N-WASP accumulation and disappearance with high time resolution. Time is given in seconds. Scale bars: 3 µm (A) and 1 µm (B).