Fig. 1. Patching of GM1-CT-B in Jurkat cells fixed at different temperatures. (A) Jurkat T cells were PFA-fixed at the indicated temperatures and treated with either CT-B-rhodamine or CT-B-Alexa Fluor 594 followed anti-CT-B to induce patching. Single confocal equatorial sections show fluorescence in the rhodamine channel. (B) Cells treated with CT-B-Alexa Fluor 594 and patched with anti-CT-B at 37°C prior to fixation. Scale bars: 10 µm.