Fig. 3. Localisation of Lck in relation to the TfR and CD3 in Jurkat cells at 0°C. Jurkat T cells were fixed at 0°C and stained with mouse anti-Lck and rabbit anti-TfR or rabbit anti-Lck and mouse anti-CD3, followed by anti-mouse-Alexa Fluor 594 and anti-rabbit-FITC or anti-rabbit-Alexa Fluor 488 antibodies. Scale bars: 5 µm.