Fig. 6. Correlation of tyrosine phosphorylation and GM1 distribution. (A) Jurkat T cells were fixed at the indicated temperatures, permeabilised and stained with CT-B-Alexa Fluor 594 and 4G10 (anti-pTyr) followed by anti-mouse FITC-conjugated secondary antibody. Single equatorial confocal sections show fluorescence in the rhodamine and FITC channels. Scale bars: 5 µm. For cells fixed at 0°C, the Pearson correlation coefficient between GM1-CT-B and tyrosine phosphorylation at the plasma membrane was 0.488±0.032 (mean±s.e.m.; n=10).