Fig. 8. Genetic requirement for cold-induced tyrosine phosphorylation. Jurkat-derived cell lines J.RT3-T3.5 (TCR/CD3 deficient) and JCam-1.6 (Lck deficient) were examined for cold-induced tyrosine phosphorylation. Cells were incubated at the indicated temperatures for 10 minutes. Lysates were analysed by western blotting for tyrosine phosphorylation (4G10). Gels were loaded on an equal protein basis, confirmed by probing for ERK (anti-p44/42 MAP kinase). For the J.RT3-T3.5 cells, the two panels correspond to different exposure times of the same membrane. Molecular mass markers are indicated (kDa). Fluorographs shown are representative of four experiments.