Fig. 9. Temperature dependence of tyrosine phosphorylation in CD4+ human primary T cells. Cells were incubated at the indicated temperatures for 10 minutes, of which, anti-CD3 (OKT3) at 1 µg/ml was present for the last 5 minutes in the stimulated aliquots (lanes +). Lysates were analysed by western blotting for (A) tyrosine phosphorylation (4G10) or (B) ERK activation (anti-phospho-p44/42 MAP kinase). Gels were loaded on an equal protein basis. Molecular mass markers are indicated (kDa). Fluorographs shown are representative of three (A) and two (B) experiments.