Fig. 1. Comparison of the sequences of Fzo/Mfn and structure of mammalian Mfn2. (A) The sequences were aligned by the ClustalW program. A black or gray background indicates that amino acid residues of all or four of the five highly homologous proteins are identical, respectively. m, Mus musculus; d, Drosophila melanogaster; c, Caenorhabditis elegans; TM, transmembrane. (B) Domain structure of mouse Mfn2. C, predicted coiled-coil domain; gray, GTPase domain; black, predicted transmembrane domains. Numbers indicate amino acid residue positions at the beginning and end of domains. Vertical lines in the lower scheme represent conserved amino acid residues. The GTPase domain is highly conserved among species. The GenBank accession numbers of the proteins are: mMfn1 (NP_077162