Fig. 2. Distribution of hnRNPs A2 and B1 in Colo16 cells at different cell cycle stages. Cells collected at S, G2, M and G1 phases were immunostained with a rabbit hnRNP A2 antibody, which also recognizes the B1 isoform, and visualized using a FITC-conjugated donkey secondary antibody. A monoclonal -tubulin antibody raised in mouse was used to counterstain microtubules with a Cy3-conjugated goat secondary antibody (red in merged images). DAPI was used to stain DNA (blue). Bar, 10 µm.