Fig. 5. hnRNP A/B mRNA levels in different cells. Real-time PCR was carried out to quantify the amount of A1, A2, B1 and A3 mRNAs in the selected cell lines using ß-actin as a control. The relative abundance of individual mRNA, shown above the columns, was calculated as the ratio between the targeted and ß-actin mRNAs. Means±s.e.m. of three experiments, each with two replicates, were calculated for each type of cell. Col, Colo16; HeL, HeLa; Ker, keratinocytes.