Fig. 4. Random recovery of GFP:NtMAP65-1a signal over the microtubule bundle length. (A) The first measurement was taken just before the photobleaching and corresponds to point 0 (the image of the microtubule at this time point is represented in part B point 0). The grey sector represents the duration of photobleaching, after which 35 images were collected and measured at approximately 2.5-second intervals. The fluorescence signal on the chart is expressed in arbitrary values. (B) Time series (left to right) of GFP:NtMAP65-1a signal recovery after photobleaching the microtubules shown in A. The numbers at the top of each image indicate time in seconds when each of the frames was collected with 0 corresponding to the image before the photobleaching onset and 12.0 just after the photobleaching. The images shown follow the microtubule to complete recovery and this occurred after 34.5 seconds. (C) Changes in time (x axes) of the distribution of the fluorescence signal (z axes) along the length of the microtubule (y axes) shown in A and B. The signal intensity is expressed in arbitrary values.