Fig. 4. NCoR and Pit-1 are co-localized within the nucleus of mouse pituitary GHFT1-5 cells. Immunocytochemical staining was used to compare the subnuclear distribution of the endogenous NCoR and Pit-1 in fixed GHFT1-5 cells. GHFT1-5 cells probed with anti-Pit1 antibody followed by Texas-Red-conjugated anti-rabbit antibody, and anti-NCoR antibody followed by FITC-conjugated anti-goat antibody. The profile analysis shows the degree of co-localization of the immunostained proteins. The specificity of staining was confirmed by analysis of cells stained with both secondary antibodies alone, each individual primary antibody with the opposite secondary antibody, or each primary with both secondary antibodies.