Fig. 5. Pit-1 redistributes NCoR in the nucleus. (A) GHFT1-5 cells were transfected with GFP-Pit-1 and PML-RFP, and the living cells were stained briefly with H33342 DNA dye immediately before imaging. (B) GHFT1-5 cells were co-transfected with GFP-NCoR, BFP-Pit-1 and PML-RFP, and the living cells were imaged using WFM. The square ROI in the overlay image is enlarged to highlight the subnuclear protein organization. Cyan in the overlay images indicates colocalization of the green and blue channels. The profile plots display the relative intensity of the fluorophores at the positions along the yellow line in the overlay images. The labeled bars indicate scale.