Fig. 8. The Pit-1 DNA-binding domain is required to disperse SMRT. GHFT1-5 cells were co-transfected with expression vectors encoding YFP-SMRT and the indicated BFP fusion protein. (A,B) The profile plots display the relative intensity of the fluorophores at the positions along the yellow line in the overlay images. (C,D) Cells were selected using co-transfected mRFP and subcellular features were quantified using integrated image analysis. The focus size and enrichment factor were normalized for the relative level of YFP-SMRT expression in each cell. The data are shown as the means of each cell population with error bars denoting s.e.m.