Fig. 1. Localization of P2Y1 in rat duodenum and in cultured subepithelial fibroblasts. Cryosections of 6W Wistar rat duodenum were incubated with (A) rabbit anti-P2Y1 antibody and (B) anti-P2Y1 antibody pre-absorbed with 2 µg ml^-1 antigenic peptide, followed with anti-rabbit Envision+ and visualized with DAB-H₂O₂ reactions. (C) Subepithelial fibroblasts isolated from rat duodenal villi in primary cultures were incubated with rabbit anti-P2Y1 antibody, then biotinylated anti-rabbit IgG, followed with streptavidin-Texas red. Bar in A and B is 100 µm, and in C is 10 µm.