Fig. 8. Suppression of Ca²⁺ responses to touch and stretch in the stellate-shape cells. Stellate-shaped cells treated with 1 mM dBcAMP for 1.5 hours did not respond to touch (A1) and stretch (B1), although an extremely strong stimulation that punctured cells induced Ca²⁺ waves (A2). After 10 minutes treatment with 10 nM ET1, cells changed to flat, and sensitivity to touch and stretch was recovered (A3,B2). Arrows indicate touched cells. Ca²⁺ increases are shown by superimposed images: (A) F405 is red, F480 is green, Nomarski is gray; and (B) F405 is red, F480 is green, ratio of F405/F480 is blue. Similar results were obtained in over 10 dishes in several different cultures in both A and B. Bars, 100 µm.