Fig. 9. Suppression of stretch-induced ATP-release in stellate-shaped cells. Subepithelial fibroblasts cultured on elastic chambers were treated with 1 mM dBcAMP for about 1 hour to induce the cells to change shape to stellate. During stretching, ATP contents in perfusates were measured every minute. (A) A typical example involving a strong stretch (32%, 40%) induces a small ATP release even in stellate-shaped cells. After ET1 (but not substance-P treatment) ATP release is obviously enhanced. (B) The average data for the effects of dBcAMP treatment and ET1 application on ATP release are shown, which were normalized by controls. n=5, bars show s.e.m.