Fig. 3. Autoinhibitory domain interactions regulate UNC-43 abundance in the neurites. Cell bodies and neurites expressing (A,B) wild-type UNC-43::GFP, (C,D) UNC-43(D238R)::GFP, (E,F) UNC-43(R283E)::GFP and (G,H) UNC-43(D238R, R283E)::GFP. Mutations in D238 result in the failure of UNC-43 to accumulate throughout the unlocalized neurite pool (D), whereas mutations in R283 (F) result in the overabundance of UNC-43 in this pool. The phenotype of the two mutations combined (H) is similar to that of D238R alone (D). (I,J) Fluorescence quantification measuring the mean fluorescent intensity values for neuron cell bodies (I) and neurites (J) expressing the indicated UNC-43::GFP construct. (K) The number of clusters (clusters defined as above a threshold of two standard deviation values of the mean outside of the clustered signal) per 10 µm length of neurite. WT indicates the wild-type UNC-43 protein, and an amino acid substitution number identifies each mutant form of UNC-43 tested. The catalytic domain, identified as UNC-43(1-270), and the subunit association domain, identified as UNC-43(271-520) were also tested. A.U., arbitrary units. Error bars indicate s.e.m. Significant differences were observed ^**P<0.01, ^***P<0.001 compared to levels in the WT groups using one-way ANOVA followed by Dunnett's t-test comparisons. n=10-25 for each genotype. Bar, 5 µm.