Fig. 2. Normal PTS1 protein localization in Hppex20 cells. (A,B) Ultrathin sections of WT and pex20 cells were used for immunolabeling experiments using antibodies against the PTS1 protein alcohol oxidase (A,B) or the PTS2 protein amine oxidase (C,D). Both in WT (A) and in pex20 cells (B) AO labeling is found at the peroxisomal profiles. AMO is normally localized to peroxisomes in WT cells (2C), but mislocalized to the cytosol in pex20 cells (D). Bars, 0.5 µm. M, mitochondrion; N, nucleus, P, peroxisome; V, vacuole.