Fig. 6. Examples of experimental autocorrelation curve (dots), fitted (solid line), and residuals (upper inset) of HpPex20p. HpPex20p was labeled with Alexa Fluor 488. In all experiments the concentration of HpPex20p was 50 nM. After global analysis of 20 experimental curves, a diffusion time of 223 microseconds was obtained (confidence limit: 218-277 microseconds). Based on these data the calculated molecular mass of the fluorescent protein complex is estimated to amount 198 kDa (confidence limit: 185-380 microseconds) assuming that the complex is globular in shape.