Fig. 7. rp3 involvement in SATB1-mediated transcriptional repression activity and gene regulation of Bcl2. (A) HEK cells were transfected with pGL2-5Gal, pTK-RL plus the indicated plasmids. The luciferase activity was measured and that of pcDNA3.1-transfected cells was taken to be 100% (column 5). (B) Luciferase assays of HEK cells transfected with pTK-RL, pGL2-5Gal, pGal4DB-SATB1 and rp3 siRNA or control siRNA. The luciferase activity obtained from cells transfected with pcDNA3.1 and treated with control siRNA was taken to be 100%. (C) rp3 reversed SATB1 suppression effect on Bcl2-MAR. Luciferase assays were performed using lysates of HEK cells transfected with pTK-RL, Bcl2-MAR-Luc and plasmid combination as indicated. The activity of cells transfected with pRK5 was taken as 100%. (D) Bcl2 mRNA levels were measured by real-time RT-PCR using RNAs isolated 24 hours after transfection with the indicated plasmids. The Bcl2 mRNA level of pRK5-transfected cells was taken to be 100%. Significant differences in luciferase activity and mRNA levels were observed (^**P<0.01) in indicated cells compared to levels in the control. All values represent the mean±s.e.m. of three separate experiments.