Fig. 1. (A) Suspension and re-adhesion of quiescent HKs activates leading keratinocytes for a wound model. HKs were grown at confluence to generate quiescence. Suspension with trypsin/EDTA activated the keratinocytes. Re-adhesion onto surfaces coated with laminin 5 was mediated by integrins 6ß4 and 3ß1. Re-adhesion was followed by cell spreading via integrin 3ß1. Spreading, but not adhesion, was blocked with Cytochalasin D. (B) cDNA microarray analysis of quiescent and suspended/re-adherent HKs. Levels of mRNA transcripts in quiescent and suspended/re-adherent HKs were compared by cDNA microarray analysis 1.5 hours post suspension/re-adhesion with spreading. Solid bars represent transcript levels of activated suspended/re-adherent HKs, whereas open bars represent transcript levels in quiescent cells. Transcript levels are reported as mean (±s.e.m.) fluorescent units (y-axis). Significant differences ^**P<0.05 and ^*P<0.10 in fluorescence levels were found between the groups indicated and the control. Microarray analysis was performed in five separate suspension/re-adhesion experiments.