Fig. 9. Orientation and localization of myofibrils in the vicinity of cell contacts and focal contacts in embryonal CAR^+/+ and CAR^-/- cardiomyocytes. (A) Indirect immunofluorescence of CAR^+/+ cells (+/+) for CAR and of CAR^+/+ cells and CAR^-/- cells (–/–) for ZO-1. (B-D) Double staining of CAR^+/+ and CAR^-/- cells for: (B) N-cadherin (green) and ß-catenin (red); (C) ß₁-integrin (green) and -actinin (red); (D) N-cadherin (green) and -actinin (red). In B and C the single stainings and the merge are shown (as indicated) whereas in D only the merged picture is depicted. Bars in A-C represent 50 µm, bar in D is 10 µm.