Fig. 4. Interaction of -Syn and PP2A in cells and in rat brain. (A) -Syn PC12 cell lysates were co-immunoprecipitated and proteins were separated by SDS-PAGE and the western blots (WB) were reacted with -Syn antibody (-S WB) or PP2A antibody (PP2A WB). Left blot shows the levels of -Syn in cells from the initial homogenate (lane 1), in the homogenate after co-immunoprecipitation (co-IP; lane 2), and -Syn immunoprecipitated with the Syn-1 antibody (lane 3). Right blot in A was prepared from the same co-IP sample with the levels of PP2A in the initial homogenate (lane 1), PP2A in the homogenate after co-IP (lane 2), and PP2A co-immunoprecipitated along with -Syn using the Syn-1 antibody (lane 3). Non-specific bands, two of which appear to be IgG bands (asterisks) are evident in both blots. (B) The -Syn WB reveals -Syn immunoprecipitated from rat striatum (left lane) and in the PP2A WB the PP2A that co-immunoprecipitated with -Syn using the Syn-1 antibody (right lane). Relative molecular mass (M_r, x10^-3) was determined from prestained standards. -S WB, -synuclein Syn-1 antibody-reacted western blot; PP2A WB, PP2A antibody-reacted western blot.