Fig. 4. Mstn inhibits the migration of SCs and macrophages. (A) Graph showing the chemo-inhibitory effect of Mstn on myoblasts. The addition of a chemoattractant to the bottom well of a blind-well chamber increases the migration of myoblasts across the filter by 200%. When Mstn was added with the chemoattractant (5 µg/ml), the migration is reduced by 50%. Mstn alone does not inhibit chemokinesis (^**P<0.01; Student's t-test). (B) Graph showing the chemo-inhibitory effect of Mstn on macrophages using blind-well chambers. The addition of a chemoattractant to the bottom well increases the migration of macrophages across the filter by over 200%. When Mstn was added with the chemoattractant (5 µg/ml), the migration is reduced by 50%. Mstn alone does not inhibit chemokinesis. (^**P<0.01; Student's t-test).