Fig. 6. HGF inhibits Mstn expression. (A) Quantitative RT-PCR was performed for Mstn and Hgf on regenerating and wild-type muscle. The amplicons were detected by Southern blot hybridization. GAPDH was used to show equal amount of RNA used. (B) C2C12 cells stably transfected with the 1.6 kb Mstn promoter luciferase reporter construct were treated with either 20 ng of HGF or with vehicle, and the luciferase assay was performed on cell lysates. Luciferase activity is normalized to ß-galactosidase activity. HGF treatment significantly downregulates Mstn promoter activity. (^***P<0.001; Student's t-test).