Fig. 3. Specific interaction between AGAP2 and AP-1. (A) Redistribution of AP-1 by AGAP2. HeLa cells were transfected with FLAG-tagged AGAP1 or AGAP2 for 24 hours. The cells were fixed and stained with antibodies against the subunit of AP-1. The transfected cells were detected using polyclonal anti-FLAG antibody. (B) Membrane dissociation of AP-3 by AGAP1. HeLa cells were transfected with FLAG-AGAP1 or AGAP2 for 24 hours. Cells were stained for the epitope tag and AP-3 using anti- antibody. (C) No effect of AGAP2 on the staining pattern of COPI or TGN46. HeLa cells were transfected with FLAG-AGAP2. The COPI coat was visualized by staining with antibody against ßCOP (panels a, b). The TGN was visualized by staining with an antibody against TGN46 (panels c, d). Transfected cells were indicated by arrows.