Fig. 9. Inhibitory phosphorylation of Cdk1 is required for infective tube formation. (A) Mixtures of the strains indicated were spotted on MMNH4-charcoal (non-inducing) and MMNO3-charcoal (inducing) plates, and incubated for 48 hours at room temperature. Fuzziness (gray colonies) was an indication of successful infective tube formation. (B) Morphology of the solopathogenic strain SG200 and its derivatives, UMC14 (SG200 Pnar1:cdk1) and UMC13 (SG200Pnar1:cdk1AF), which were incubated in inducing conditions (liquid MMNO3 medium) for 8 hours. Scale bars: 20 µm.