Fig. 1. dVps16A physically interacts with Dor and Car. (A) Cytosol from S2 cells was fractionated by sucrose density gradient centrifugation (10-20%). Proteins in each fraction were detected by western blotting with the indicated antibodies. Sedimentation coefficients (S20w) are indicated for two markers. (B) Proteins were immunoprecipitated from S2-cell cytosol with anti-Dor, anti-Car, anti-dVps16A, or anti-Boss antibodies as controls. Immunoprecipitates were detected on western blots with the indicated antibodies to Dor, dVps16 or Car.