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Fig. 7. ApsB accumulates at the hyphal tip in dynein-mutant cells. Cytoplasmic, MT-associated ApsB (white spots) often localizes at the hyphal tip as one single spot. This is independent of whether apsB is tagged N- or C-terminally with GFP (A,B) (SEa3, SDM1000). The localization pattern was not changed, neither in a {Delta}kinA (C) (SDM64) nor in a {Delta}kipA (D) (SDV19) background. However, up to 10 ApsB-GFP dots accumulated in {Delta}nudA strains (E) (SDM92). Despite their accumulation at the hyphal tip, each spot was still highly mobile (see supplementary material, Movies 17 and 18). (F) ApsB localization in apsA1 mutant stains (SDV31). Scale bar is 3 µm for (A-D,F) and 2 µm for (E).