JCS -- Gontier et al. 118 (16): 3739 Figure IG3

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Fig. 3. FATZ-1 forms a complex with myotilin in vitro. ³⁵S-labeled HA-tagged recombinant form of FATZ-1 (A) and FATZ-2 (C) were generated by coupled in vitro transcription/translation and analyzed by SDS-PAGE and autoradiography. GST-fusion proteins of myotilin immobilized on glutathione-agarose beads were incubated with either ³⁵S-labeled FATZ-1 (B) or FATZ-2 (D). Arrows indicate the protein of interest. After washing, bound proteins were analyzed by SDS-PAGE and autoradiography. Molecular mass markers are indicated in kDa.