Fig. 9. Binding of the C-terminal region of filamin-C with the cytoplasmic domain of the ß1A integrin subunit in yeast two-hybrid assays and pull-down experiments. (A) +, growth; –, no growth, on selective media in yeast two-hybrid assays, tested as described in Materials and Methods and Fig. 4. Although filamin-C (19-21) and filamin C (20-21) bound to ß1A, filamin C (21-23) did not. (B) Detection by immunoblotting of the expressed products from cDNA constructs for HA-tagged filamin-C (19-21) and filamin-C (21-23) in lysates of transiently transfected COS-7 cells. Pull-down assay with GST-ß1A or GST immobilized on glutathione beads containing either HA-tagged filamin C (19-21) (C) or filamin-C (21-23) (D) fusion proteins. Immunoblot detection of bound filamin-C (19-21) to GST-ß1A. No binding was observed with filamin-C (21-23).