Fig. 2. Effects of growth factors on ESC-derived VEGFR2⁺ cells. (A-D) ESC-derived VEGFR2⁺ cells were treated with growth factors for 1.5 days, followed by immunostaining with anti-PECAM1 (green) and anti-SMA (red). Cells were treated with (A) a combination of VEGF-A (30 ng/ml) and FGF-2 (10 ng/ml; represented by a gradation of green and light blue in this and subsequent figures), (B) VEGF-A (green), (C) FGF-2 (light blue), or (D) PDGF-BB (20 ng/ml; red). Scale bars: 100 µm. (E) Rates (%) of appearance of PECAM1⁺ cells (green bars) and SMA⁺ cells (red bars). Error bars represent the standard deviation (s.d.). Corresponding video microscopy recordings taken for a half-day prior to fixation can be seen in Movies 1-4 in supplementary material.