Fig. 6. Matrigel plug assay with other types of combined stimulation. (A,B) Combined stimulation with VEGF-A (200 ng/ml) plus PDGF-BB (1 µg/ml) (A, V/P treatment) and FGF-2 (1 µg/ml) plus PDGF-BB (1 µg/ml) (B, F/P treatment). Red arrows and orange arrowheads in H and E stained section (first row) show mature blood vessels and vessels with extravascular RBCs, respectively. White arrowheads and yellow arrows in immunohistochemically stained sections (second and third row) indicate detached SMA⁺ cells and PECAM1⁺ cells not covered by SMA⁺ cells, respectively. Arrows in the permeability-assay section (fourth row) indicate leakage of dextran. Scale bars: 60 µm in H and E stained sections: 20 µm in immunohistochemically stained sections. (C,D) Newly formed vessels and vessel-like structures were quantified using the same method as in Fig. 5. *P<0.05, **P<0.01. Corresponding figures for NG2 and desmin staining are shown in Fig. S4 in supplementary material.