Fig. 4. SCAMPs on the plasma membrane. Plasma membrane sheets were labeled for SCAMP1 (A), SCAMP2 (B) or transferrin receptor (TfR) (C). (D) EM image of a portion of plasma membrane sheet ripped off the upper surface of an NRK cell and double labeled with antibodies against SCAMP1 and 2 conjugated directly to 5nm and 10 nm colloidal gold. Asterisk indicates clathrin-coated patch. Staining of SCAMP1 (E-G) and SCAMP2 (H-J) on plasma membrane sheets is also compared to clathrin (clat, E,H), adaptin AP2 (F,I) and dynamin 2 (dyn2, G,J). Insets illustrate limited colocalization. Bar, 10 µm (C); 100 nM (D).