Fig. 5. SCAMPs are not detected in post-Golgi carriers for VSV-G. NRK cells transfected with GFP-VSVG were incubated at 39°C for 4 hours, then at 20°C for 2 hours to accumulate VSV-G in the TGN (A-C) and were warmed to 32°C for 30 minutes in the presence of 0.5% tannic acid to trap VSV-G in secretory carriers prevented from fusing with the plasma membrane (D-F). Deconvolved sections of cells immunostained for SCAMP1 (B,E) and SCAMP4 (C,F) compare the distributions of the SCAMPs to GFP-VSV-G. Bar, 10 µm.