Fig. 9. The effect of Eps15-DPF expression and of methyl-ß-cyclodextrin on the distribution of constitutively recycling cargo and SCAMP1. COS-7 cells were transiently transfected with GFP-tagged Eps15-DPF, fixed, immunostained for SCAMP1 or TfR and visualized by fluorescence (A-D) or TIRF-M (G-H) to compare SCAMP or TfR staining to GFP in paired images. (E,F) COS-7 cells were treated with 10 mM methyl-ß-cyclodextrin (CD, 30 minutes, 37°C), fixed, double-labeled for SCAMP1 and TfR, and examined by fluorescence. Bar, 10 µm.