Fig. 1. Localisation of CLIP-190 in the cell cycle. S2 cells were immunostained with CLIP-190 and -tubulin antibodies, DNA was counterstained with DAPI. (A) In interphase cells, CLIP-190 is mainly localised to microtubule plus-ends. (B) Magnified view of the region marked by the rectangle in A. Three arrowheads indicate the variation in CLIP-190 localisation at microtubule plus-ends, which may reflect differences in microtubule dynamics. (C) In prometaphase, CLIP-190 is localised to kinetochores. (D) In metaphase, CLIP-190 disappears from kinetochores although it still associates weakly with spindle microtubules. Bars, 10 µm.